Description
Principle of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-GDNF antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-GDNF antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDNF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDNF can be calculated.
Background: Glial cell-derived neurotrophic factor, also known as GDNF, is a founding member of the GDNF family of ligands (GFL). In humans, it is encoded by the GDNF gene. This gene mapped to 5p13.3-p13.1. Mutations in this gene may be associated with Hirschsprung's disease. GDNF is a small protein that potently promotes the survival of many types of neurons. The most prominent feature of GDNF is its ability to support the survival of dopaminergic and motorneurons. It promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. It also regulates kidney development and spermatogenesis, and it affects alcohol consumption